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Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). The frost-free season is comparatively long for a location with such severe winters. The last spring frost is on average around 23 May, whilst the first fall frost is on 22 September. Each stage of development in the diagram is paired with a photomicrograph of a developing P. aeruginosa biofilm. All photomicrographs are shown to the same scale. Each parent must decide whether or not to stay and care for their offspring, or to desert their offspring. This decision is best modeled by game theoretic approaches to evolutionarily stable strategies (ESS) where the best strategy for one… Clones varied in BbbqrA expression, with one clone displaying a more than eightfold increase in BbbqrA transcript levels compared with the wild-type parent (Fig. 2 B and C). This strain was used in all subsequent studies reported below.

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